Ribonucleotide Reductase Overproduction in Hydroxyurea Resistant Mouse Cells.
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منابع مشابه
Hydroxyurea-resistant vaccinia virus: overproduction of ribonucleotide reductase.
Repeated passages of vaccinia virus in increasing concentrations of hydroxyurea followed by plaque purification resulted in the isolation of variants capable of growth in 5 mM hydroxyurea, a drug concentration which inhibited the reproduction of wild-type vaccinia virus 1,000-fold. Analyses of viral protein synthesis by using [35S]methionine pulse-labeling at intervals throughout the infection ...
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Ribonucleotide reductase (EC 1.17.4.1 -2) catalyses the direct reduction of ribonucleotides to the corresponding deoxyribonucleotides. This reaction enabled the evolution from the early ‘RNA world’ to the present ‘DNA world’. Storage of genetic information as DNA instead of RNA is much more stable and this was a prerequisite for the later development of multiple, separate, species [l] . Ribonuc...
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A somatic mutant cell line of murine T-lymphoma cells (S49), dGuo-200-1, has been isolated and contains a ribonucleotide reductase (Ullman, B., Clift, S. M., Gudas, L. J., Levinson, B. B., Wormsted, M. A., and Martin, D. W., Jr. (1980) J. Biol. Chem 255,8308-8314) with altered allosteric properties. The enzyme in dGuo200-1 extracts was inhibited only 50% by the allosteric inhibitor, dATP. Thus,...
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Mammalian ribonucleotide reductase is a highly regulated activity essential for DNA synthesis and repair. The activity and message levels of the enzyme are elevated in cells treated with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate, and this appears to be mediated through specific cis elements in the 3'-untranslated region of the R1 and R2 mRNAs that interact with R1 and R2 binding p...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1980
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.34b-0152